Dr. Redmond Smyth
Über
Nach dem naturwissenschaftlichen Studium an der Universität Cambridge (Großbritannien) wechselte Redmond Smyth für seine Doktorarbeit an das Burnet Institute, Melbourne (Australien), um die Mechanismen der genetischen Vielfalt von HIV-1 in seinen natürlichen Zielzellen zu untersuchen. In seiner Doktorarbeit wies er auf die Bedeutung der RNA-Struktur bei der Regulierung viraler Infektionsprozesse hin. Als Postdoc am Institut Biologie Moléculaire et Cellulaire (IBMC) des Centre national de la recherche scientifique (CNRS) und der Universität Straßburg (Frankreich) ließ er sich zum RNA-Biochemiker ausbilden. Dabei untersuchte er die Mechanismen, die zum Einbau der genomischen HIV-1-RNA in Viruspartikel führen. Als Chargé de recherche (CR2) forschte er von 2015 an weiter am IBMC. 2018 gründete er seine eigenständige Helmholtz-Nachwuchsgruppe am HIRI.
2024
NEAT1 promotes genome stability via m6A methylation-dependent regulation of CHD4
Mamontova V, Trifault B, Gribling-Burrer AS, Bohn P, Boten L, Preckwinkel P, Gallant P, Solvie D, Ade CP, Papadopoulos D, …, Smyth RP, Burger K (2024)
Genes & Development 38 (17-20): 915-930DOI: 10.1101/gad.351913.124
Recruitment of multi-segment genomic RNAs by Bluetongue virus requires a preformed RNA network
Sung PY, Phelan JE, Luo D, Kulasegaran-Shylini R, Bohn P, Smyth RP, Roy P (2024)P., Roy P (2024)
Nucleic Acids Research 52 (14): 8500-8514DOI: 10.1093/nar/gkae404
Sequencing accuracy and systematic errors of nanopore direct RNA sequencing
Liu-Wei W, van der Toorn W, Bohn P, Hölzer M, Smyth RP, von Kleist M (2024)
BMC Genomics 25 (1): 528DOI: 10.1186/s12864-024-10440-w
Isoform-specific RNA structure determination using Nano-DMS-MaP
Gribling-Burrer AS, Bohn P, Smyth RP (2024)
Nature Protocols 19 (6): 1835-1865DOI: 10.1038/s41596-024-00959-3
2023
SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9
Schmidt N, Ganskih S, Wei Y, Gabel A, Zielinski S, Keshishian H, Lareau CA, Zimmermann L, Makroczyova J, Pearce C, …, Erhard F, Munschauer M (2023)
Cell 186 (22): 4834-4850.e23DOI: 10.1016/j.cell.2023.09.002
Advanced fluorescence microscopy in respiratory virus cell biology
Xie E, Ahmad S, Smyth RP, Sieben C (2023)
Advances in Virus Research 116: 123-172DOI: 10.1016/bs.aivir.2023.05.002
Sequential disruption of SPLASH-identified vRNA-vRNA interactions challenges their role in influenza A virus genome packaging
Jakob C, Lovate GL, Desirò D, Gießler L, Smyth RP, Marquet R, Lamkiewicz K, Marz M, Schwemmle M, Bolte H (2023)
Nucleic Acids Research 51 (12): 6479-6494DOI: 10.1093/nar/gkad442
Nano-DMS-MaP allows isoform-specific RNA structure determination
Bohn P, Gribling-Burrer AS, Ambi UB, Smyth RP (2023)
Nature Methods 20 (6): 849-859DOI: 10.1038/s41592-023-01862-7
Cis-mediated interactions of the SARS-CoV-2 frameshift RNA alter its conformations and affect function
Pekarek L, Zimmer MM, Gribling-Burrer AS, Buck S, Smyth RP, Caliskan N (2023)
Nucleic Acids Research 51 (2): 728–743DOI: 10.1093/nar/gkac1184
2022
Short- and long-range interactions in the HIV-1 5' UTR regulate genome dimerization and packaging
Ye L, Gribling-Burrer AS, Bohn P, Kibe A, Börtlein C, Ambi UB, Ahmad S, Olguin-Nava M, Smith M, Caliskan N, von Kleist M, Smyth RP (2022)
Nature Structural & Molecular Biology 29 (4): 306-319DOI: 10.1038/s41594-022-00746-2
2021
RNA Structures and Their Role in Selective Genome Packaging
Ye L, Ambi UB, Olguin-Nava M, Gribling-Burrer AS, Ahmad S, Bohn P, Weber MM, Smyth RP (2021)
Viruses 13 (9): 1788DOI: 10.3390/v13091788
The short isoform of the host antiviral protein ZAP acts as an inhibitor of SARS-CoV-2 programmed ribosomal frameshifting
Zimmer MM, Kibe A, Rand U, Pekarek L, Ye L, Buck S, Smyth RP, Cicin-Sain L, Caliskan N (2021)
Nature Communications 12 (1): 7193DOI: 10.1038/s41467-021-27431-0
2019
The evolution of RNA structural probing methods: From gels to next-generation sequencing
Mailler E, Paillart J, Marquet R, Smyth RP, Vivet-Boudou V (2019)
Wiley Interdisciplinary Reviews: RNA 10 (2): e1518DOI: 10.1002/wrna.1518
2018
RNA Structure - A Neglected Puppet Master for the Evolution of Virus and Host Immunity
Smyth RP, Negroni M, Lever AM, Mak J, Kenyon JC (2018)
Frontiers in Immunology 9: 2097DOI: 10.3389/fimmu.2018.02097
Structural and Functional Motifs in Influenza Virus RNAs
Ferhadian D, Contrant M, Printz-Schweigert A, Smyth RP, Paillart J, Marquet R (2018)
Frontiers in Microbiology 9: 559DOI: 10.3389/fmicb.2018.00559
In cell mutational interference mapping experiment (in cell MIME) identifies the 5' polyadenylation signal as a dual regulator of HIV-1 genomic RNA production and packaging
Smyth RP, Smith MR, Jousset A, Despons L, Laumond G, Decoville T, Cattenoz P, Moog C, Jossinet F, Mougel M, …, Kleist M, Marquet R (2018)
Nucleic Acids Research 46 (9): e57DOI: 10.1093/nar/gky152
2017
HIV-1 Pr55Gag binds genomic and spliced RNAs with different affinity and stoichiometry
Bernacchi S, Abd El-Wahab EW, Dubois N, Hijnen M, Smyth RP, Mak J, Marquet R, Paillart J (2017)
RNA Biology 14 (1): 90-103DOI: 10.1080/15476286.2016.1256533
2016
HIV-1 Mutation and Recombination Rates Are Different in Macrophages and T-cells
Cromer D, Schlub TE, Smyth RP, Grimm AJ, Chopra A, Mallal S, Davenport MP, Mak J (2016)
Viruses 8 (4): 118DOI: 10.3390/v8040118
A step forward understanding HIV-1 diversity
Smyth RP, Negroni M (2016)
Retrovirology 13: 27DOI: 10.1186/s12977-016-0259-8
The Life-Cycle of the HIV-1 Gag-RNA Complex
Mailler E, Bernacchi S, Marquet R, Paillart J, Vivet-Boudou V, Smyth RP (2016)
Viruses 8 (9): E248DOI: 10.3390/v8090248
MIMEAnTo: profiling functional RNA in mutational interference mapping experiments
Smith MR, Smyth RP, Marquet R, Kleist M (2016)
Bioinformatics 32 (21): 3369-3370DOI: 10.1093/bioinformatics/btw479
2015
Evaluation of anti-HIV-1 mutagenic nucleoside analogues
Vivet-Boudou V, Isel C, El Safadi Y, Smyth RP, Laumond G, Moog C, Paillart J, Marquet R (2015)
The Journal of Biological Chemistry 290 (1): 371-83DOI: 10.1074/jbc.M114.616383
Properties of HIV-1 associated cholesterol in addition to raft formation are important for virus infection
Hawkes D, Jones KL, Smyth RP, Pereira CF, Bittman R, Jaworowski A, Mak J (2015)
Virus Research 210: 18-21DOI: 10.1016/j.virusres.2015.06.023
Mutational interference mapping experiment (MIME) for studying RNA structure and function
Smyth RP, Despons L, Huili G, Bernacchi S, Hijnen M, Mak J, Jossinet F, Weixi L, Paillart J, Kleist M, Marquet R (2015)
Nature Methods 12 (9): 866-72DOI: 10.1038/nmeth.3490
2014
Identifying recombination hot spots in the HIV-1 genome
Smyth RP, Schlub TE, Grimm AJ, Waugh C, Ellenberg P, Chopra A, Mallal S, Cromer D, Mak J, Davenport MP (2014)
Journal of Virology 88 (5): 2891-902DOI: 10.1128/JVI.03014-13
Fifteen to twenty percent of HIV substitution mutations are associated with recombination
Schlub TE, Grimm AJ, Smyth RP, Cromer D, Chopra A, Mallal S, Venturi V, Waugh C, Mak J, Davenport MP (2014)
Journal of Virology 88 (7): 3837-49DOI: 10.1128/JVI.03136-13
Specific recognition of the HIV-1 genomic RNA by the Gag precursor
Abd El-Wahab EW, Smyth RP, Mailler E, Bernacchi S, Vivet-Boudou V, Hijnen M, Jossinet F, Mak J, Paillart J, Marquet R (2014)
Nature Communications 5: 4304DOI: 10.1038/ncomms5304
2013
Improved quantification of HIV-1-infected CD4+ T cells using an optimised method of intracellular HIV-1 gag p24 antigen detection
Yang H, Yorke E, Hancock G, Clutton G, Sande N, Angus B, Smyth RP, Mak J, Dorrell L (2013)
Journal of Immunological Methods 391 (1-2): 174-8DOI: 10.1016/j.jim.2013.03.001
Intracellular Dynamics of HIV Infection
Petravic J, Ellenberg P, Chan M, Paukovics G, Smyth RP, Mak J, Davenport MP (2013)
Journal of Virology 88 (2): 1113-24DOI: 10.1128/JVI.02038-13
A functional sequence-specific interaction between influenza A virus genomic RNA segments
Gavazzi C, Yver M, Isel C, Smyth RP, Rosa-Calatrava M, Lina B, Moulès V, Marquet R (2013)
PNAS 110 (41): 16604-9DOI: 10.1073/pnas.1314419110
2012
The Origin of Genetic Diversity in HIV-1
Smyth RP, Davenport MP, Mak J (2012)
Virus Research 169 (2): 415-29DOI: 10.1016/j.virusres.2012.06.015
2011
8-Modified-2'-deoxyadenosine analogues induce delayed polymerization arrest during HIV-1 reverse transcription
Vivet-Boudou V, Isel C, Sleiman M, Smyth RP, Ben Gaied N, Barhoum P, Laumond G, Bec G, Götte M, Mak J, …, Burger A, Marquet R (2011)
PLOS One 6 (11): e27456DOI: 10.1371/journal.pone.0027456
Early events of HIV-1 infection: can signaling be the next therapeutic target?
Jones KL, Smyth RP, Pereira CF, Cameron PU, Lewin SR, Jaworowski A, Mak J (2011)
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 6 (2): 269-83DOI: 10.1007/s11481-011-9268-5
Labeling of multiple HIV-1 proteins with the biarsenical-tetracysteine system
Pereira CF, Ellenberg PC, Jones KL, Fernandez TL, Smyth RP, Hawkes DJ, Hijnen M, Vivet-Boudou V, Marquet R, Johnson I, Mak J (2011)
PLOS One 6 (2): e17016DOI: 10.1371/journal.pone.0017016
2010
Accurately measuring recombination between closely related HIV-1 genomes
Schlub TE, Smyth RP, Grimm AJ, Mak J, Davenport MP (2010)
PLOS Computational Biology 6 (4): e1000766DOI: 10.1371/journal.pcbi.1000766
Reducing chimera formation during PCR amplification to ensure accurate genotyping
Smyth RP, Schlub TE, Grimm A, Venturi V, Chopra A, Mallal S, Davenport MP, Mak J (2010)
Gene 469 (1-2): 45-51DOI: 10.1016/j.gene.2010.08.009
2009
The A-rich RNA sequences of HIV-1 pol are important for the synthesis of viral cDNA
Keating CP, Hill MK, Hawkes DJ, Smyth RP, Isel C, Le S, Palmenberg AC, Marshall JA, Marquet R, Nabel GJ, Mak J (2009)
Nucleic Acids Research 37 (3): 945-56DOI: 10.1093/nar/gkn1015